Analysis of apoptosis mechanism in SGC-7901 induced by CSBE with flow cytometry and confocal laser scanning techenology

Flow cytometry is a laser based, biophysical technology employed in cell counting, sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. Confocal laser scanning microscopy is a technique for obtaining high-resolution optical images with depth selectivity. The flow cytometry and confocal laser scanning microscopy were used to study the changes of initiator, effector and marker in process of mitochondrial apoptosis pathway induced by CSBE. In addition, the regulation genes of apoptosis were surveyed with western blotting and flow cytometry. As a result, the mechanism of apoptosis induced by CSBE was to increase the expression of pro-apoptotic protein Bax, decrease the expression of anti-apoptotic protein Bcl-2, which induced mitochondrial PTP channel open, decreased mitochondrial membrane potential, led to release of mitochondrial Cyt-c and caused activation of caspase-3. To sum up, the mitochondrial apoptosis pathway of SGC-7901 induced by CSBE was revealed by the flow cytometry and confocal laser scanning microscopy.