Title :
Aptamer biosensors for microorganism detection
Author :
Congxiao Zhang ; Xuefei Lv ; Hong Qing ; Lina Geng ; Yulin Deng
Author_Institution :
Sch. of Life Sci., Beijing Inst. of Technol., Beijing, China
Abstract :
Aptamers are single-stranded oligonucleotides that bind with high specificity and affinity to a target molecule. Aptamer-based biosensors have become a new class of biosensors, which utilized aptamers as highly selective recognition elements as a result of good performance in the capture of target analytes. In this study, aptamer was used as an recognition element of biosensor, and was integrated to microarray chip to realized the detection of L. achidophilus. The fluorescence and transmission image of bacterial cells were obtained in different times magnification field. The results demonstrated that the aptamer biosensor based on microarray chip can be used to detect L. achidophilus by means of FITC labeled bacterial cells.
Keywords :
DNA; biosensors; cellular biophysics; fluorescence; lab-on-a-chip; microorganisms; molecular biophysics; FITC labeled bacterial cells; L. achidophilus detection; aptamer-based biosensors; binding; fluorescence image; high selective recognition elements; magnification field; microarray chip; microorganism detection; single-stranded oligonucleotides; target analytes; target molecule; transmission image; Arrays; Biosensors; DNA; Fluorescence; Glass; Microorganisms; Microscopy; Lactobacillus acidophilus; biosensor; microorganism detection;
Conference_Titel :
Complex Medical Engineering (CME), 2013 ICME International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4673-2970-5
DOI :
10.1109/ICCME.2013.6548250