Fragmentation pattern of glycated peptides derived from D-glucose by tandem mass spectrometry

Evidence shows that non-enzymatic glycation plays an important role in the development of diabetes, diabetes-related complications and neurodegenerative diseases. Mass spectrometric methods have been used for non-enzymatic glycation of proteins. At present, CID remains the major fragmentation method for peptide sequencing. In this study, we utilized synthesized peptide models to study fragmentation spectra of glycated peptides, which can be easily studied using ESI-MS. MS/MS fragmentation whose characteristics of glycated peptides will help for large-scale glycated proteomics analyses of complex plasma and tissue samples.