Ultrasound contrast agents mediated cell labeling for MRI tracking

Author

Skachkov, I. ; Ying Luan ; van Tiel, Sandra ; van der Steen, Ton ; Kooiman, K. ; Bernsen, Monique ; de Jong, Nico

Author_Institution

Dept. of Biomed. Eng., Thoraxcenter, Erasmus MC, Rotterdam, Netherlands

fYear

2012

fDate

7-10 Oct. 2012

Firstpage

2278

Lastpage

2281

Abstract

In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ~7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.

Keywords

biomedical MRI; biomedical ultrasonics; blood vessels; cellular biophysics; ultrasonic imaging; MRI tracking; cell viability; endothelial cells; frequency 1 MHz; in vivo cell tracking; inflammation; metastasis; optimal ultrasound parameters; pressure 40 kPa; stem cell tracking; time 5 min; ultrasound contrast agent-mediated SPIO-labeling; ultrasound contrast agents mediated cell labeling; Acoustics; Drugs; In vitro; In vivo; Labeling; Magnetic resonance imaging; Ultrasonic imaging; MRI; SPIO; cell tracking; targeted microbubbles; ultrasound contrast agents;

fLanguage

English

Publisher

ieee

Conference_Titel

Ultrasonics Symposium (IUS), 2012 IEEE International

Conference_Location

Dresden

ISSN

1948-5719

Print_ISBN

978-1-4673-4561-3

Type

conf

DOI

10.1109/ULTSYM.2012.0569

Filename

6562427