Title :
Laminar Optical Tomography Applied to Reporter Genes Imaging in Engineered Tissue Constructs
Author :
Ozturk, Mehmet S. ; Lee, Vivian K. ; Lingling Zhao ; Guohao Dai ; Intes, Xavier
Author_Institution :
Biomed. Eng. Dept., Rensselaer Polytech. Inst., Troy, NY, USA
Abstract :
Fluorescence imaging is the main method in assessing tissue engineered constructs. However, with the drive to develop thick engineered constructs, it is imperative to develop new fluorescence based techniques that can image beyond the depth capabilities of current microscopic techniques. Here we report the use of Laminar Optical Tomography (LOT) to image in 3D vascular channels, formed by GFP and mCherry carrier cells, in a thick printed constructs enclosed in a perfusion chamber. Results show the potential of LOT for locating and monitoring transfected cells in thick tissue with spatial accuracy.
Keywords :
biological tissues; biomedical materials; biomedical optical imaging; cellular biophysics; fluorescence; genetics; optical tomography; tissue engineering; 3D vascular channel; GFP; LOT potential; depth capability; fluorescence based technique; fluorescence imaging; laminar optical tomography; mCherry carrier cells; microscopic technique; perfusion chamber; reporter gene imaging; thick engineered construct; thick printed construct; tissue engineered construct; transfected cell locating; transfected cell monitoring; Biomedical optical imaging; Fluorescence; Microscopy; Optical imaging; Optical microscopy; Tomography; 3D reconstruction; mesoscopic; reporter genes; tissue engineering; tomographic;
Conference_Titel :
Bioengineering Conference (NEBEC), 2013 39th Annual Northeast
Conference_Location :
Syracuse, NY
Print_ISBN :
978-1-4673-4928-4
DOI :
10.1109/NEBEC.2013.106
