Title :
Analysis and Characterization of HK-2 Cells for High Throughput Drug Testing
Author :
Sakolish, Courtney ; Reiss, Matthew ; Mahler, Grant
Author_Institution :
Dept. of Biomed. Eng., Binghamton Univ., Binghamton, NY, USA
Abstract :
We are working to develop a multi-channel microfluidic device to model human kidney function using HK-2 (human kidney proximal tubule) cells as a model cell type. Optimization of growth conditions and characterization of cells was achieved through the use of various methods including growth curves, measurement of epithelial electrical resistance, enzymatic assays, immunohistochemistry, and transport studies. These studies allowed for us to understand the growth of renal cells over time, formation of tight junctions between cells, modes of transport and permeability of these cells to proteins. The results of the characterization studies suggest that HK-2 cells can be used to model human proximal tubule function in pharmaceutical testing.
Keywords :
bioMEMS; cellular transport; kidney; physiological models; HK-2 cell analysis; HK-2 cell characterization; HK-2 cells; cell permeability; cell protein transport modes; enzymatic assay; epithelial electrical resistance; growth condition optimization; high throughput drug testing; human kidney function modeling; human kidney proximal tubule cells; human proximal tubule function; immunohistochemistry; model cell type; multichannel microfluidic device; pharmaceutical testing; renal cell growth; tight intercellular junction formation; Biomedical engineering; HK-2; Human Kidney; Pharmaceutical Testing;
Conference_Titel :
Bioengineering Conference (NEBEC), 2013 39th Annual Northeast
Conference_Location :
Syracuse, NY
Print_ISBN :
978-1-4673-4928-4
DOI :
10.1109/NEBEC.2013.52