Title :
Electroporation system generating wide range square-wave pulses for biological applications
Author :
Stankevic, Voitech ; Novickij, Vitalij ; Balevicius, Saulius ; Zurauskiene, Nerija ; Baskys, Algirdas ; Dervinis, A. ; Bleizgys, V.
Author_Institution :
Center for Phys. Sci. & Technol., Vilnius Gediminas Tech. Univ., Vilnius, Lithuania
fDate :
Oct. 31 2013-Nov. 2 2013
Abstract :
The design of a wide range square-wave pulse electroporation system is presented. The system is able to generate pulses having voltage amplitudes up to 4000 V and currents up to 100 A. The quantity of accumulated energy is optimized by the use of the variable capacitor bank. The proposed device has an integrated graphic LCD and measurement modules for current pulse visualization, and does not require an external oscilloscope. The whole system was tested with suspension of human lymphoma cells of Jurkat line in RPMI medium under a variety of electroporation conditions.
Keywords :
biological techniques; capacitors; cellular biophysics; liquid crystal displays; Jurkat line; RPMI medium; accumulated energy; biological applications; capacitor bank; current pulse visualization; electroporation conditions; human lymphoma cells; integrated graphic LCD; voltage amplitudes; wide range square-wave pulse electroporation system; Biology; Capacitors; Energy storage; Resistance; Suspensions; Transistors; Voltage measurement;
Conference_Titel :
Biomedical Circuits and Systems Conference (BioCAS), 2013 IEEE
Conference_Location :
Rotterdam
DOI :
10.1109/BioCAS.2013.6679633
