Title :
Application of chemiluminescence involving flow injection analysis to determination of glucose, glutamic acid and lactic acid for food analysis
Author :
Xing Wang ; Ishii, Naohiro ; Ming Ye ; Munkhjargal, Munkhbayar ; Miyajima, Kiyotomi ; Arakawa, Takeshi ; Saito, Hiroshi ; Kudo, Hiroyuki ; Endo, Hiroshi ; Mitsubayashi, Kohji
Author_Institution :
Inst. of Biomater. & Bioeng., Tokyo Med. & Dental Univ., Tokyo, Japan
Abstract :
A simple application of chemiluminescence (CL) to multicomponent analysis of glucose, glutamic acid and lactic acid for food analysis using flow injection analysis (FIA) is described. The oxidative enzyme for each analyte and horseradish peroxidase (HRP) was employed. The sample solutions were analysed by the catalytic reaction on luminol/H2O2 CL reaction system. The CL generated by catalytic reactions was detected by a FIA system consisted of a photodiode, enzyme-immobilized reactor, a peristaltic pump and a personal computer. Glucose oxidase (GOD), glutamate oxidase (GLOD) and lactate oxidase (LOD) was immobilized on the hydrophilic polytetrafluoroethylene membrane, respectively. A small volume, 5.0 μL of the standard solution of each analyte was injected and the CL was detected and measured by a photodiode. And the measurement of glucose, glutamic acid and lactic acid is concurrently also possible by using GOD, GLOD and LOD enzyme-immobilized reactors.
Keywords :
bioreactors; enzymes; food processing industry; membranes; organic compounds; sugar; CL; FIA; GLOD; GOD; HRP; LOD; analyte; catalytic reaction; chemiluminescence; enzyme-immobilized reactor; flow injection analysis; food analysis; glucose; glucose oxidase; glutamate oxidase; glutamic acid; horseradish peroxidase; hydrophilic polytetrafluoroethylene membrane; lactate oxidase; lactic acid; luminol; multicomponent analysis; oxidative enzyme; peristaltic pump; photodiode; Biochemistry; Biomedical measurement; Inductors; Photodiodes; Standards; Sugar;
Conference_Titel :
SENSORS, 2013 IEEE
Conference_Location :
Baltimore, MD
DOI :
10.1109/ICSENS.2013.6688378