Slope and cantilever type electrode fabrication for measurement of neurospheroid signal

Cell therapy has been studied a lot to apply to cranial nerve degenerative diseases. But overcoming limitation of low survival rate of transplanted cells is the important thing for cell therapy. For this, studies of transplanting cells in spheroid type are under way. However, the characteristic of cell spheroid is not grasped yet. General MEA can get signals only on a single contact spot with neurospheroid, it is hard to grasp the character of cells spheroid. For this, 3D slope type and cantilever type electrode were developed to make multiple contacts possible with cell spheroid. In electrochemical impedance of fabricated electrodes, slope type electrode was 80.5 kohm, and cantilever type electrode was 16 kohm per 1kHz on the average. In contact impedance, slope type was 422.96 kohm, and cantilever type was 46 kohm. It is considered to be suited enough for nerve signal measurement of neurospheroid in the future research.