New facile method for preparing themperature-resopnsive cell culture surfaces using a thioxantone-based photoinitiator immobilized polystyrene surface

Author

Fukumori, Kazuhiro ; Tanaka, Nobuyuki ; Ota, Hiroyuki ; Akiyama, Yoko ; Itoga, Kazuyoshi ; Yamato, Masayuki ; Okano, Teruo

Author_Institution

Instn. for Adv. Biomed. Sci., Tokyo Women´s Med. Univ., Tokyo, Japan

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

1

Lastpage

3

Abstract

PIPAAm grafted polystyrene (PIPAAm-Pst) dishes were prepared using visible light irradiation and polystyrene dishes modified with thioxanethone-based photoinitiator. At optimized preparation conditions, PIPAAm-PSs fabricating cell sheets were successfully prepared by 5 mins visible light irradiation. After PIPAAm polymerization, micro-patterned temperature responsive polystyrene culture surface (MPTRCS) were generated by additional micro-patterned polymerization of PAAm. Endothelial cells were attached selectively PIPAAm region at 37°C. By lowing temperature, micro-pattered endothelial cells were detached from MPTRCS as continuously micro-pattered cell sheet.

Keywords

biochemistry; cellular biophysics; materials preparation; polymer blends; polymerisation; radiation effects; MPTRCS; PIPAAm grafted polystyrene; PIPAAm-PS fabricating cell sheets; micropatterned PIPAAm polymerization; micropatterned endothelial cell sheet; micropatterned temperature responsive polystyrene culture surface; optimized preparation conditions; polystyrene dishes; temperature 37 degC; themperature-resopnsive cell culture surfaces; thioxanethone-based photoinitiator; thioxantone-based photoinitiator immobilized polystyrene surface; time 5 min; visible light irradiation; Biomedical imaging; Bovine; Fibroblasts; Polymers; Radiation effects; Solvents; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-1527-9

Type

conf

DOI

10.1109/MHS.2013.6710390

Filename

6710390