Sensitivity compensation of multi-fluorescence sensor toward on-chip cell measurement

Author

Hengjun Liu ; Maruyama, Hisataka ; Masuda, T. ; Arai, Fumihito

Author_Institution

Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

1

Lastpage

2

Abstract

We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it is independent to PH. The relative fluorescence intensity of FITC can respond to both pH and temperature. We succeeded in the compensation of the pH sensitivity of FITC using temperature measurement by Rhodamine B.

Keywords

biosensors; cellular biophysics; dyes; fluorescence; lab-on-a-chip; microsensors; optical sensors; pH; pH measurement; polymers; proteins; sensitivity; temperature measurement; FITC; Rhodamine B; amino-polystyrene microbeads; fluorescent indicators; multifluorescence sensor; on-chip cell measurement; pH response; pH sensitivity; relative fluorescent intensity; sensitivity compensation; temperature measurement; temperature response; Calibration; Equations; Fluorescence; Polymers; Sensitivity; Temperature measurement; Temperature sensors;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-1527-9

Type

conf

DOI

10.1109/MHS.2013.6710415

Filename

6710415