Double-strand breaks on a genomic DNA caused by ultrasound: Evaluation by single DNA observation

Author

Kenmotmsu, Takahiro ; Ogawa, Naoko ; Kubota, R. ; Yoshida, Kenta ; Kagawa, Yasuhiro ; Watanabe, Yoshihiro ; Yoshikawa, Yasuhiro ; Yoshikawa, Kenichi

Author_Institution

Fac. of Life & Med. Sci., Doshisha Univ., Kyotanabe, Japan

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

1

Lastpage

3

Abstract

Double-strand brakes of T4-DNA molecules caused by ultrasound irradiation are monitored through the methodology of single giant DNA observation by fluorescence microscopy. The experimental results indicate the existence of the threshold power of ultrasound on the double-strand brakes. In addition, we have examined the effect of an anti-oxidative agent, 2-mercaptoethanol (2-Me), on the ultrasound damage of DNA. It becomes clear that 2-Me exhibits almost no protective effect.

Keywords

DNA; biochemistry; biological effects of acoustic radiation; biomolecular effects of radiation; fluorescence; genomics; optical microscopy; oxidation; 2- mercaptoethanol; T4-DNA molecules; antioxidative agent; double-strand breaks; fluorescence microscopy; genomic DNA; single giant DNA observation; ultrasound damage; ultrasound irradiation; Cavity resonators; Chemicals; DNA; Fluorescence; Microscopy; Radiation effects; Ultrasonic imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2013 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-1527-9

Type

conf

DOI

10.1109/MHS.2013.6710461

Filename

6710461