Title :
Optimization of illumination frequency and preclinical validation of a wide-field structured illumination microscope designed for imaging in situ tumor margins
Author :
Fu, Henry L. ; Mueller, Jennifer L. ; Javid, Melodi ; Kirsch, David G. ; Ramanujam, Nimmi ; Brown, J. Quincy
Author_Institution :
Dept. of Biomed. Eng., Duke Univ., Durham, NC, USA
Abstract :
We present a widefield structured illumination microscope for imaging surgical tumor margins in situ. The impact of frequency and turbidity on optical section thickness and SNR was characterized to determine the optimal imaging frequency.
Keywords :
biomedical optical imaging; fluorescence; optical microscopy; surgery; tumours; turbidity; frequency impact; illumination frequency optimization; in situ surgical tumor imaging margins; optical section thickness; optimal imaging frequency; preclinical validation; turbidity impact; wide-field structured illumination microscopy; Biomedical optical imaging; Fluorescence; Lighting; Microscopy; Optical imaging; Tumors;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
