Title :
Depth-resolved nanoscopic single particle tracking based on fluorescence phase-shifting interferometry
Author :
Arbel, Eli ; Bilenca, Alberto
Author_Institution :
Biomed. Eng. Dept., Ben-Gurion Univ. of the Negev, Beer-Sheva, Israel
Abstract :
We propose and analyze the use of fluorescence phase-shifting interferometric (PSI) imaging for single particle tracking along 1 μm-depth (z) trajectories. While spatial PSI using a single camera attains a maximal twofold improvement in the z-localization precision compared to temporal PSI for <;0.1 μm2/s diffusion, temporal PSI shows a fourfold larger field-of-view.
Keywords :
cameras; fluorescence; image sensors; optical tracking; phase shifting interferometry; depth 1 mum; depth trajectories; depth-resolved nanoscopic single particle tracking; field-of-view; fluorescence phase-shifting interferometric imaging; fluorescence phase-shifting interferometry; maximal twofold improvement; single camera; spatial PSI; temporal PSI; z-localization precision; Cameras; Fluorescence; Nanobioscience; Particle tracking; Photonics; Trajectory;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2013 Conference on
Conference_Location :
San Jose, CA
