Biocompatibility of human Whartons Jelly Mesenchymal Stem Cells on poly-caprolactone and collagen based nanofiber mats

Nanofiber scaffolds were fabricated to analyze the proliferation of human Whartons Jelly Mesenchymal Stem Cells (hWJMSCs) for skin tissue engineering applications. Poly-caprolactone (PCL) and PCL mixed with collagen scaffolds were fabricated using electrospinning. ImageJ analysis was carried out on SEM images to characterize the structural and morphological properties of the nanofiber scaffolds. Tensile testing was also performed to quantify the mechanical properties of the scaffolds. The scaffolds were then seeded with hWJMSCs to study cell proliferation over five days in order to determine the feasibility for tissue regeneration. The average fiber diameters for the PCL scaffold and for the PCL/collagen scaffold were 0.542 μm and 0.633 μm, respectively. The Young´s Moduli of the PCL and PCL/collagen scaffolds were 0.00370 Pa and 0.00683 Pa. respectively. After analyzing the data, it can be concluded that the PCL/collagen scaffold is better suited for regeneration of damaged or diseased nervous tissue.