Direct characterization of radial modulus of DNA nanotube by AFM nanoindentation

DNA origami in a nanotube configuration is increasingly attractive for the creation of new structural systems that can confine or encapsulate a variety of nanoscale materials, but utilization as a functional mechanical component depends on understanding crucial properties such as the radial mechanical property that has rarely been studied. Here, we report the direct characterization of the radial modulus of a DNA nanotube by AFM nanoindentation. A corrected Hertz model was employed to interpret the measured force versus distance data. The biphasic material properties of the DNA nanotube were characterized for the first time and indicate a radial electrostatic modulus of about 10 ± 3 MPa in the small region where force was applied, and a radial elastic modulus of about 63 ± 20 MPa in a larger region.