Title :
Rotating magnetic field induced mechanical stress and cell death on magnetically labeled cell
Author :
Huang, C. ; Wei, Z.
Author_Institution :
Inst. of NanoEngineering & Microsyst., Nat. Tsing Hua Univ., Tainan, Taiwan
Abstract :
The nonuniformity mechanical force can induce changes in the membrane structure of biological cell and lead to cell death. In this study, three magnetic nanoparticle (MNPs) labeled cells (3T3, SKHep-1, SiHa)were subjected to rotating magnetic field with different field strength(2-15 Hz), frequency and treatments were last for different periods . Quantitative measurement analyzed MNPs uptake of three cells before the exposure of rotating magnetic field . Pruissian blue staining visualizes the location of magnetic nanoparticles and MTT colorimetric assay was used to assessed cell viability . Results suggests the number of internalized MNPs show good correlation with rotating field induced mechanical stress; besides, frequency exceed a certain value show less effects the viability .
Keywords :
colorimetry; internal stresses; magnetic particles; nanoparticles; MTT colorimetric assay; biological cell; cell death; cell viability; field strength; frequency 2 Hz to 15 Hz; magnetic nanoparticle labeled cells; magnetically labeled cell; mechanical force; membrane structure; pruissian blue; rotating field induced mechanical stress; rotating magnetic field induced mechanical stress; Cities and towns; Magnetic field measurement; Magnetic fields; Magnetomechanical effects; Nanobioscience; Nanoparticles; Stress;
Conference_Titel :
Magnetics Conference (INTERMAG), 2015 IEEE
Conference_Location :
Beijing
Print_ISBN :
978-1-4799-7321-7
DOI :
10.1109/INTMAG.2015.7156954
