Title :
A 64 pixel ISFET-based biosensor for extracellular pH gradient monitoring
Author :
Nabovati, Ghazal ; Ghafar-Zadeh, Ebrahim ; Sawan, Mohamad
Author_Institution :
Dept. of Electr. Eng., Polytech. Montreal, Montreal, QC, Canada
Abstract :
We present an integrated biosensor platform using ion-sensitive field effect transistor (ISFET) device for monitoring pH levels in cell culture medium. Each pixel in the 8×8 array employs a pair of p-type and n-type ISFETs sharing the same ion sensitive membrane. The complementary pair of ISFETs works as a chemical switch that can be used for pH thresholding. The proposed biosensor is fabricated using TSMC 0.35 μm standard CMOS technology. We show the functionality and sensitivity of device by using buffer solutions with different pH values. The proposed biosensor is functional in the wide pH range of 1 to 14 with the sensitivity of 50 mV/pH. Due to its low complexity, pH sensitivity, and biocompatibility, this platform can be used for cellular studies applications such as cancer cell growth monitoring.
Keywords :
CMOS integrated circuits; biomembranes; biosensors; cancer; cellular biophysics; chemical sensors; ion sensitive field effect transistors; pH; ISFET-based biosensor; TSMC standard CMOS technology; buffer solutions; cancer cell growth monitoring; cell culture medium; extracellular pH gradient monitoring; integrated biosensor platform; ion sensitive membrane; ion-sensitive field effect transistor device; size 0.35 mum; Arrays; Chemicals; Inverters; Sensitivity; Sensors; Switches; Threshold voltage; Biosensor; CMOS; Cell growth monitoring; ISFET;
Conference_Titel :
Circuits and Systems (ISCAS), 2015 IEEE International Symposium on
Conference_Location :
Lisbon
DOI :
10.1109/ISCAS.2015.7168995
