Title :
Osmotic erythrocyte lysis for chemical- and label-free impedance cytometry
Author :
Winkler, T.E. ; Ben-Yoav, H. ; Kelly, D.L. ; Ghodssi, R.
Author_Institution :
Fischell Dept. of Bioeng., Univ. of Maryland, College Park, MD, USA
Abstract :
We present the first implementation of osmotic erythrocyte (RBC) lysis for microfluidic leukocyte (WBC) impedance cytometry. The method relies on osmotic pressure differentials to rupture RBC membranes, and detection of WBCs based on changes in impedance. Specifically, we demonstrate an up to 1,200-fold decrease in noise from RBCs in WBC recordings. Our approach enables lab-on-a-chip (LOC)-based WBC counting on whole blood samples without traditionally required chemicals or labels.
Keywords :
biomembranes; blood; cellular biophysics; lab-on-a-chip; microfluidics; osmosis; RBC membrane rupture; lab-on-a-chip-based WBC counting; microfluidic leukocyte impedance cytometry; osmotic erythrocyte lysis; osmotic pressure differentials; whole blood samples; MATLAB; Mathematical model; Microsystem integration; impedance cytometry; lab-on-a-chip; microfluidics; osmotic lysis;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7180933
