Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

Author

Nakano, M. ; Ding, Z. ; Kasahara, H. ; Suehiro, J.

Author_Institution

Kyushu Univ., Fukuoka, Japan

fYear

2015

fDate

21-25 June 2015

Firstpage

1530

Lastpage

1532

Abstract

This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

Keywords

DNA; biomedical electrodes; electrochemical impedance spectroscopy; electrophoresis; microelectrodes; molecular biophysics; dielectric microbeads; dielectrophoresis; dielectrophoretic impedance spectroscopy; frequency dependent impedance; microelectrode; polymerase chain reaction amplified DNA; rapid size determination; DNA; Dielectrophoresis; Frequency measurement; Impedance; Impedance measurement; Microelectrodes; Spectroscopy; DNA amplification; DNA length; DNA size separation; dielectrophoresis; microbeads;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181228

Filename

7181228