DocumentCode :
731953
Title :
A rapid on-chip single nucleotide polymorphism detection directly from human blood
Author :
Zhang, L. ; Cai, Q. ; Jones, B. ; Fiorini, P. ; Stakenborg, T. ; Masahiko, T. ; Lagae, L.
Author_Institution :
imec, Leuven, Belgium
fYear :
2015
fDate :
21-25 June 2015
Firstpage :
1669
Lastpage :
1671
Abstract :
This paper reports on a silicon/glass based microsystem for the fast detection of single nucleotide polymorphisms (SNPs) directly from on-chip filtered human blood. A crossflow micro-pillar filter is used for purifying lysed blood. It provides an easy solution for on-chip blood sample preparation. After purification, polymerase chain reaction (PCR) was no longer inhibited. The purified DNA solution is then tested for the detection of specific SNPs in a thermally insulated micro-cavity by real-time PCR. As a proof of principle, a successful on-chip detection of two SNPs in human CYP2C9 gene is demonstrated. The SNP profiling using our device took less than 40 minutes from sample to result.
Keywords :
DNA; bioMEMS; biochemistry; biological specimen preparation; biomedical measurement; blood; enzymes; filtration; genetics; glass; lab-on-a-chip; microfluidics; molecular biophysics; polymorphism; purification; real-time systems; silicon; thermal insulation; SNP profiling; Si; crossflow micropillar filter; direct SNP detection; human CYP2C9 gene; lysed blood purification; on-chip blood sample preparation; on-chip filtered human blood; polymerase chain reaction; purified DNA solution testing; rapid on-chip single nucleotide polymorphism detection; real-time PCR; silicon-glass based microsystem; thermally insulated microcavity; Blood; Cavity resonators; DNA; Inhibitors; Probes; Real-time systems; System-on-chip; Microfluidic; genotyping; lab on a chip; polymerase chain reaction; single nucleotide polymorphism;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
Type :
conf
DOI :
10.1109/TRANSDUCERS.2015.7181263
Filename :
7181263
Link To Document :
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