Floating-flower inspired cell culture platform for simplifying medium exchange based on elasto-capillarity

Author

Hong, H. ; Park, S.J. ; Na, M.H. ; Park, S.M. ; Kim, D.S.

Author_Institution

Dept. of Mech. Eng., POSTECH, Pohang, South Korea

fYear

2015

fDate

21-25 June 2015

Firstpage

1857

Lastpage

1860

Abstract

In this study, Flower-Shaped Polydimethylsiloxane (PDMS) Substrate (FSPS) motivated from a floating flower was proposed to simplify a cell culture experiment for studying an effect of cell microenvironments. The FSPS can capture and dispense a cell culture medium, forming a pocket by physical interaction between an elastic substrate and the medium. Thus, we can exchange the medium with simple lifting up and pushing down actions without repetitive pipetting. The developed platform was verified to substitute a conventional PDMS substrate utilized in the studies, through stability and sustainability of the medium pocket, and NIH3T3 cell proliferation experiments.

Keywords

bioMEMS; biological techniques; capillarity; cellular biophysics; microfluidics; polymers; substrates; FSPS; NIH3T3 cell proliferation experiment; cell culture experiment; cell culture medium capture; cell culture medium dispensing; cell microenvironment effect; conventional PDMS substrate; elastic substrate-medium physical interaction; elasto-capillarity; floating-flower inspired cell culture platform; flower-shaped polydimethylsiloxane substrate; medium exchange simplification; medium pocket stability; medium pocket sustainability; Assembly; Curing; Substrates; Surface morphology; Surface topography; Surface treatment; Cell culture substrate; Cell microenvironment; Floating flower; Medium exchange;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181311

Filename

7181311