Lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel

Author

Gupta, Arpan ; Bonde, Shital R. ; Gaikwad, Sameer ; Ingle, Atul ; Gade, Aniket K. ; Rai, Mahendra

Author_Institution

Dept. of Biotechnol., Sant Gadge Baba Amravati Univ., Amravati, India

Volume

8

Issue

3

fYear

2014

fDate

Sept. 2014

Firstpage

172

Lastpage

178

Abstract

Lawsonia inermis mediated synthesis of silver nanoparticles (Ag-NPs) and its efficacy against Candida albicans, Microsporum canis, Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag-NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO₃. The Ag-NPs were characterized by Ultraviolet-Visible (UV-Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag-NPs. The antimicrobial activity of Ag-NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag-NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag-NPs may be an answer to the drug-resistant microorganisms.

Keywords

Fourier transform spectra; antibacterial activity; biochemistry; biodiffusion; biomedical materials; cellular biophysics; drugs; gels; infrared spectra; microorganisms; nanofabrication; nanomedicine; nanoparticles; reduction (chemical); silver; transmission electron microscopy; ultraviolet spectra; visible spectra; Ag; C. albicans; Candida albicans; Fourier transform infrared spectroscopy; L. inermis; Lawsonia inermis mediated synthesis; M. canis; Microsporum canis; P. acne; Propioniabacterium acne; T. mentagrophytes; Trichophyton mentagrophytes; UV-vis spectrophotometer; UV-visible spectrophotometer; antibiotics; antimicrobial activity; antimicrobial nanogel formulation; antimicrobial nanoparticle gel; bactericidal agents; bioreduction; biosynthesis route; capped nanoparticle formation; chemical agents; disc diffusion method; drug-resistant microorganisms; fungicidal agents; human pathogenic fungi; intermediate complex formation; leaf extract; microbial resistance; nanoparticle tracking; silver nanoparticles; transmission electron microscopy; zeta potential;

fLanguage

English

Journal_Title

Nanobiotechnology, IET

Publisher

iet

ISSN

1751-8741

Type

jour

DOI

10.1049/iet-nbt.2013.0015

Filename

6869129