Multiscale modelling of coupled Ca2+ channels using coloured stochastic Petri nets

Stochastic modelling of coupled Ca²⁺ channels is a challenge, especially when the coupling of the channels, as the authors address this problem using coloured stochastic Petri nets (SPN^C) as high-level description to generate determined by their spatial arrangement relative to each other, has to be considered at multiple spatial scales. In this study, continuous-time Markov chains. The authors develop several models with increasing complexity. They first apply SPNC to model single clusters of coupled Ca²⁺ channels arranged in a regular or irregular lattice, where they describe how to represent the geometrical arrangement of Ca²⁺ channels relative to each other using colours. They then apply this modelling idea to construct more complex models by modelling spatially arranged clusters of channels. The authors´ models can be easily reproduced and adapted to different scenarios.