A Wireless Bio-MEMS Sensor for C-Reactive Protein Detection Based on Nanomechanics

Author

Chen, Chun-Hao ; Hwang, Rong-Zhang ; Huang, Long-Sun ; Lin, Shi-Ming ; Chen, Hsiao-Chin ; Yang, Yu-Che ; Lin, Yu-Tso ; Yu, Shih-An ; Lin, Yo-Sheng ; Wang, Yiao-Hong ; Chou, Nai-Kuan ; Shey-Shi Lu

Author_Institution

Grad. Inst. of Electron. Eng., Nat. Taiwan Univ., Taipei

Volume

56

Issue

2

fYear

2009

Firstpage

462

Lastpage

470

Abstract

A quick wireless label-free detection of disease-related C-reactive proteins (CRPs) using a 200-mum-long microelectromechanical systems (MEMS) microcantilever housed in a 7times7 mm² reaction chamber with a safe reusable feature is reported. The assay time ranges from about 30 min to 3 h, depending on accuracy. The deflection of the microcantilever due to specific CRP-antiCRP binding is detected using a position-sensitive detector. The converted bio-signal is transmitted by a custom designed wireless amplitude-shift-keying (ASK) transceiver IC fabricated in a 0.18 mum CMOS process. CRP concentrations from 1 mug/mL to 500 mug/mL can be detected. A 0.2-Hz 1-V ac signal instead of traditional bases/acids is applied to the bio-MEMS sensor to unbind the CRP from the microcantilever for reusability.

Keywords

amplitude shift keying; bioMEMS; biosensors; cantilevers; lab-on-a-chip; microsensors; molecular biophysics; nanomechanics; proteins; wireless sensor networks; C-reactive protein detection; CMOS process; CRP-antiCRP binding; microcantilever; microelectromechanical systems; nanomechanics; position-sensitive detector; reaction chamber; reusability; wireless amplitude-shift-keying transceiver IC; wireless bioMEMS sensor; wireless label-free detection; Amplitude shift keying; Biosensors; CMOS integrated circuits; CMOS process; Microelectromechanical systems; Micromechanical devices; Position sensitive particle detectors; Proteins; Transceivers; Wireless sensor networks; ASK transmitter; Analog-to-digital converter (ADC); C-reactive protein (CRP); amplitude-shift-keying (ASK) receiver; antiCRP; label-free; microcantilever; microcontrol unit (MCU); poly-dimethyl-siloxane (PDMS); Biosensing Techniques; C-Reactive Protein; Cardiovascular Diseases; Dimethylpolysiloxanes; Equipment Design; Equipment Reuse; Humans; Micro-Electrical-Mechanical Systems; Monitoring, Ambulatory; Nanotechnology; Signal Processing, Computer-Assisted;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2008.2003262

Filename

4595681