ﺗﺤﺮﯾﮏ ﻫﻤﺰﻣﺎن ﺳﯿﮕﻨﺎﻟﻬﺎي ﻣﺮﺗﺒﻂ ﺑﺎ ﻇﺮﻓﯿﺖ ﻫﻮازي ﻋﻀﻠﻪ و ﺳﻨﺘﺰ ﭘﺮوﺗﺌﯿﻦ ﻣﺘﻌﺎﻗﺐ ﻓﻌﺎﻟﯿﺖ راه رﻓﺘﻦ ﺑﺎ ﻣﺤﺪودﯾﺖ ﺟﺮﯾﺎن ﺧﻮن در ﻣﺮدان ﺳﺎﻟﻢ ﺗﻤﺮﯾﻦ ﻧﮑﺮده

Concomitant Stimulation of Aerobic Capacity and Protein Synthesis Related Signaling after Walking with Blood Flow Restriction in Untrained Healthy Male

ﺳﺎﺑﻘﻪ و ﻫﺪف: ﺗﻤﺮﯾﻦ ﺑﺎ ﻣﺤﺪودﯾﺖ ﺟﺮﯾﺎن ﺧﻮن )BFR( ﺑﻪ ﺳﻤﺖ ﻋﻀﻠﻪ ﻓﻌﺎل، ﺑﻪ ﻋﻨﻮان ﯾﮏ روش ﺗﻤﺮﯾﻨﯽ ﺟﺪﯾﺪ ﻣﻮرد ﺗﻮﺟﻪ ﻣﺤﻘﻘﯿﻦ ﻗﺮار ﮔﺮﻓﺘﻪ اﺳﺖ. ﻣﻄﺎﻟﻌﺎت ﭘﯿﺸﻨﻬﺎد ﮐﺮده اﻧﺪ ﮐﻪ ﺗﻤﺮﯾﻦ BFR در ﺑﻬﺒﻮد ﻫﻤﺰﻣﺎن آﻣﺎدﮔﯽ ﻗﻠﺒﯽ ﻋﺮوﻗﯽ و ﻋﻀﻼﻧﯽ ﻣﻮﺛﺮ اﺳﺖ. ﺑﺮ اﯾﻦ اﺳﺎس، ﺑﺮاي درك ﺑﻬﺘﺮ ﻣﮑﺎﻧﯿﺴﻤﻬﺎي درﮔﯿﺮ در اﯾﻦ ﺳﺎزﮔﺎرﯾﻬﺎ، ﻫﺪف اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ ﺗﺤﺮﯾﮏ ﻫﻤﺰﻣﺎن ﺳﯿﮕﻨﺎﻟﻬﺎي ﻣﺮﺗﺒﻂ ﺑﺎ ﻇﺮﻓﯿﺖ اﺳﺘﻘﺎﻣﺘﯽ ﻋﻀﻠﻪ و ﺳﻨﺘﺰ ﭘﺮوﺗﺌﯿﻦ ﭘﺲ از ﻓﻌﺎﻟﯿﺖ راه رﻓﺘﻦ ﺑﺎ ﻣﺤﺪودﯾﺖ ﺟﺮﯾﺎن ﺧﻮن ﺑﻮد. ﻣﻮاد و روشﻫﺎ: ﭘﻨﺞ ﻣﺮد ﺳﺎﻟﻢ ﺗﻤﺮﯾﻦ ﻧﮑﺮده (ﺳﻦ: 33/4±1/02 ﺳﺎل؛ ﺗﻮده ﺑﺪن: 79/64±4/69 ﮐﯿﻠﻮﮔﺮم؛ ﻗﺪ: 173/4±9/02 ﺳﺎﻧﺘﯽ ﻣﺘﺮ؛ ﭼﺮﺑﯽ ﺑﺪن: 18/97±2/22 درﺻﺪ) در دو وﻫﻠﻪ ﺟﺪاﮔﺎﻧﻪ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ: (1) راهرﻓﺘﻦ ﺑﺎ BFR ﺑﺎ ﺷﺪت 40% از ﺗﻮان ﻫﻮازي ﺑﯿﺸﯿﻨﻪ و (2) ﻓﻌﺎﻟﯿﺖ در ﺷﺮاﯾﻂ ﮐﺎﻣﻼ ﻣﺸﺎﺑﻪ ﺑﺪون BFR. ﻧﻤﻮﻧﻪﻫﺎي ﺑﺎﯾﻮﭘﺴﯽ (ﻋﻀﻠﻪ ﭘﻬﻦ ﺟﺎﻧﺒﯽ) ﻗﺒﻞ و 3 ﺳﺎﻋﺖ ﭘﺲ از ﻓﻌﺎﻟﯿﺖ و ﻧﻤﻮﻧﻪﻫﺎي ﺧﻮﻧﯽ ﻧﯿﺰ ﻗﺒﻞ، ﺑﻼﻓﺎﺻﻠﻪ ﺑﻌﺪ و 2 ﺳﺎﻋﺖ ﺑﻌﺪ از ﻓﻌﺎﻟﯿﺖ از ﺳﯿﺎﻫﺮگ آﻧﺘﻪ ﮐﻮﺑﯿﺘﺎل ﮔﺮﻓﺘﻪ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻣﺤﺘﻮاي ﭘﺮوﺗﺌﯿﻦﻫﺎي (0/012 =PGC-1α (P و ﻓﺴﻔﻮرﯾﻼﺳﯿﻮن (0/017 =Akt (P ﺳﻪ ﺳﺎﻋﺖ ﭘﺲ از ﻓﻌﺎﻟﯿﺖ ﺑﺎ BFR ﺑﻪ ﻃﻮر ﻣﻌﻨﯽداري از ﮔﺮوه ﻓﻌﺎﻟﯿﺖ ﺑﺪون BFR ﺑﺎﻻﺗﺮ ﺑﻮد. ﻻﮐﺘﺎت ﺧﻮن و ﮐﻮرﺗﯿﺰول ﻣﺘﻌﺎﻗﺐ ﻓﻌﺎﻟﯿﺖ ﺑﺎ و ﺑﺪون BFR اﻓﺰاﯾﺶ ﻣﻌﻨﯽدار ﻧﺸﺎن ﻧﺪادﻧﺪ. 1-IGF، ﺑﻼﻓﺎﺻﻠﻪ ﺑﻌﺪ ﻓﻌﺎﻟﯿﺖ ﺑﺎ BFR در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﻣﻘﺎدﯾﺮ اوﻟﯿﻪ اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار داﺷﺖ (0/001 =P). ﻫﻮرﻣﻮن رﺷﺪ ﻧﯿﺰ ﺑﻼﻓﺎﺻﻠﻪ ﺑﻌﺪ از ﻓﻌﺎﻟﯿﺖ ﺑﺎ BFR در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﻣﻘﺎدﯾﺮ ﻗﺒﻞ از ﻓﻌﺎﻟﯿﺖ و در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﻓﻌﺎﻟﯿﺖ ﺑﺪون BFR اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار ﻧﺸﺎن داد (0/046 =P). ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺳﯿﮕﻨﺎﻟﻬﺎي ﻣﺮﺗﺒﻂ ﺑﺎ ﻋﻤﻠﮑﺮد ﻫﻮازي و ﻫﺎﯾﭙﺮﺗﺮوﻓﯽ ﻋﻀﻠﻪ ﺑﻪ ﻃﻮر ﻫﻤﺰﻣﺎن ﻣﺘﻌﺎﻗﺐ راه رﻓﺘﻦ ﺑﺎ BFR ﻓﻌﺎل ﺷﺪه و اﺣﺘﻤﺎﻻ ﺗﻮﺟﯿﻬﯽ ﺑﺮاي ﺑﻬﺒﻮد ﻋﻤﻠﮑﺮد ﻫﻮازي و ﻫﺎﯾﭙﺮﺗﺮوﻓﯽ ﻣﺸﺎﻫﺪه ﺷﺪه در ﭼﻨﺪﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻤﺮﯾﻨﯽ ﻣﺘﻌﺎﻗﺐ ﺗﻤﺮﯾﻦ ﻫﻮازي ﺑﺎ ﻣﺤﺪودﯾﺖ ﺟﺮﯾﺎن ﺧﻮن ﻣﯽ ﺑﺎﺷﻨﺪ.

Background & Purpose: Training with blood flow restriction (BFR) to the active muscle has taken to consideration of researchers as a new training method. Studies have shown that BFR training is simultaneously effective in improving cardiovascular and muscle fitness components. Accordingly, to better understand the mechanisms involved in these adaptations, the purpose of this study was to investigate of concomitant stimulation of aerobic capacity and protein synthesis related signaling after walking with blood flow restriction. Methodology: On two different occasions, five healthy untrained male subjects were asked to perform (i) a BFR walking exercise at an exercise intensity of 40 % of VO2max; and (ii) similar exercise bouts without BFR (Ctrl). For each condition, Baseline and 3-h post-exercise muscle biopsy (vastus lateralis) were sampled for protein expression analysis. Venous blood samples were also collected at baseline, immediately and 2-h post-exercise. Results: PGC-1α protein expression (P= 0.012) and Akt phosphorylation (P= 0.017) were significantly higher at 3-h post-exercise with BFR in comparison to exercise without BFR (P< 0.05). Blood lactate and serum cortisol did not significantly change. IGF-1 concentration significantly increased (P=0.001) immediately following BFR exercise than baseline values and serum GH showed a significant increase (P=0.046) compared with Ctrl. Conclusion: The results provided evidence that signaling related to aerobic capacity and hypertrophy concomitantly activated following walking exercise with BFR and likely are an explanation for improving aerobic performance and hypertrophy that observed after several previous BFR training studies.