بيان پروتئين آروماتاز در بافت چربي زيرجلدي شكمي زنان باردار مبتلا به سندرم تخمدان پلي كيستيك

Expression of Aromatase Protein in Abdominal Subcutaneous Adipose Tissue of Pregnant Women with Polycystic Ovary Syndrome (PCOS)

ﻣﻘﺪﻣﻪ: ﺳﻨﺪرم ﺗﺨﻤﺪان ﭘﻠﯽ ﮐﯿﺴﺘﯿﮏ )Polycystic ovary syndrome ﯾﺎ PCOS( ﯾﮑﯽ از اﺧﺘﻼﻻت ﻫﻮرﻣﻮﻧﯽ در زﻧﺎن اﺳﺖ. اﯾﻦ ﺳﻨﺪرم، ﻣﻤﮑﻦ اﺳﺖ در ﻧﺘﯿﺠﻪ ي ﮐﺎﻫﺶ ﻓﻌﺎﻟﯿﺖ آروﻣﺎﺗﺎز ﺑﺎﺷﺪ. ﻣﻄﺎﻟﻌﻪ ي ﺣﺎﺿﺮ، ﺑﺎ ﻫﺪف ﺑﺮرﺳﯽ ﺗﻔﺎوت ﺑﯿﺎن ﭘﺮوﺗﯿﺌﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﻧﺎن ﺑﺎردار ﻣﺒﺘﻼ ﺑﻪ ﺳﻨﺪرم ﺗﺨﻤﺪان ﭘﻠﯽ ﮐﯿﺴﺘﯿﮏ و زﻧﺎن ﺑﺎردار ﻏﯿﺮ ﻣﺒﺘﻼ اﻧﺠﺎم ﺷﺪ. روش ﻫﺎ: در ﻗﺎﻟﺐ ﯾﮏ ﻣﻄﺎﻟﻌﻪ ي ﻣﻮرد- ﺷﺎﻫﺪ، ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﺟﻠﺪي ﺑﯿﻦ 11 زن ﺑﺎردار ﻣﺒﺘﻼ ﺑﻪ PCOS و 11 زن ﻏﯿﺮ ﻣﺒﺘﻼ ﮐﻪ دو ﺑﻪ دو از ﻟﺤﺎظ ﺧﺼﻮﺻﯿﺎت وزﻧﯽ ﻗﺒﻞ از ﺑﺎرداري و ﺳﻦ ﺑﺎ ﻫﻢ ﺟﻔﺖ ﺷﺪه ﺑﻮدﻧﺪ، ﻣﻘﺎﯾﺴﻪ ﺷﺪ. ﻧﻤﻮﻧﻪ ي ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﺟﻠﺪي ﺑﻪ ﻣﯿﺰان 3-4 ﮔﺮم، ﺣﯿﻦ ﺟﺮاﺣﯽ ﺳﺰارﯾﻦ ﺑﻪ دﺳﺖ آﻣﺪ. ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ از ﻃﺮﯾﻖ وﺳﺘﺮن ﺑﻼت ارزﯾﺎﺑﯽ و ﻧﺘﺎﯾﺞ ﺑﺎ اﺳﺘﻔﺎده از ﻧﺮم اﻓﺰار SAS ﻣﻘﺎﯾﺴﻪ ﺷﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: ﻃﺒﻖ واﮐﺎوي اﻧﺠﺎم ﺷﺪه و ﺑﺎ ﮐﻤﯽ ﮐﺮدن ﻧﺘﺎﯾﺞ ﻧﺸﺎن داده ﺷﺪ ﮐﻪ ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﺟﻠﺪي ﻧﺎﺣﯿﻪ ي ﺷﮑﻤﯽ ﮔﺮوه ﻣﺒﺘﻼ ﺑﻪ PCOS ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻏﯿﺮ ﻣﺒﺘﻼ، ﮐﺎﻫﺶ ﻣﻌﻨﯽ داري داﺷﺖ )0/006 = P(. ﺑﻪ ﻋﺒﺎرت دﯾﮕﺮ، ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﯾﺮﺟﻠﺪي ﺷﮑﻤﯽ زﻧﺎن ﻣﺒﺘﻼ ﺑﻪ PCOS ﺣﺪود ﯾﮏ ﺳﻮم ﻧﺴﺒﺖ ﺑﻪ زﻧﺎن ﺑﺎردار ﻏﯿﺮ ﻣﺒﺘﻼ ﺑﻪ PCOS ﺑﯿﺎن ﮐﻤﺘﺮي داﺷﺖ. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻣﻄﺎﻟﻌﻪ ي ﮐﻨﻮﻧﯽ ﻧﺸﺎن ﻣﯽ دﻫﺪ ﮐﻪ ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ آروﻣﺎﺗﺎز در ﺑﺎﻓﺖ ﭼﺮﺑﯽ زﻧﺎن PCOS ﮐﺎﻫﺶ ﺑﯿﺎن دارد. اﯾﻦ ﯾﺎﻓﺘﻪ، ﻣﯽ ﺗﻮاﻧﺪ ﻧﻘﺶ ﮐﻠﯿﺪي ﺑﺎﻓﺖ ﭼﺮﺑﯽ در اﺧﺘﻼﻻت ﻫﻮرﻣﻮﻧﯽ زﻧﺎن ﺑﺎردار ﻣﺒﺘﻼ ﺑﻪ اﯾﻦ ﺳﻨﺪرم را ﺗﺄﯾﯿﺪ ﮐﻨﺪ.

Background: Polycystic ovary syndrome (PCOS) is one of the hormonal disorders in women. The PCOS may be due to decreased aromatase activity in adipose tissue. The aim of this study was to investigate the differences in the expression of aromatase protein in adipose tissue of pregnant women with and without PCOS. Methods: In this case-control study, aromatase protein expression were compared in subcutaneous adipose tissue of 11 pregnant women with and 11 without PCOS who were matched with each other in terms of weight characteristics before pregnancy and age. A subcutaneous adipose tissue samples (3-4 grams) was obtained during cesarean section. The expression of aromatase protein in adipose tissue was assessed using Western blotting method. All obtained data were statistically analyzed using SAS software. Findings: According to the analysis and quantification of the results, it was shown that the expression of aromatase protein in the subcutaneous adipose tissue of the abdomen of the group with PCOS decreased significantly compared to the non-PCOS group (P = 0.006). In other words, the expression of aromatase protein was about one-third lower in the adipose tissue of women with PCOS than in women without it. Conclusion: The current study shows that the expression of aromatase protein in the adipose tissue of women with PCOS is reduced. This finding could confirm the key role of adipose tissue in the development of symptoms of this syndrome.