معرفي تكنيك ثبت داخل سلولي CURRENT CLAMP به عنوان روشي جديد در بررسي اثرات مواد اندودانتيك ENDODONTIC MATERIALS

Introduction of intracellular recording technique as a novel method to investigate the effects of dental materials on excitable tissues

Background & Aim: The most detailed information about neural activity comes from intracellular recording. Not only this technique shows the pattern of neural action potential, but it also records the cellular events which give rise to the action potential, including variations in the resting potential, changes in cellular resistance and synaptic potentials. Results: Using this method with manipulation of the composition of the bathing solution (changing ionic concentrations or adding drugs) the ionic mechanisms responsible for these potentials can be identified. It is also possible to investigate the mechanisms of actions of drugs such as dental sealers on neuronal excitability. Conclusion: In the present study, intracellular recording technique has been introduced in dental materials research as a valuable method to determine the neurotoxicity of endodontic sealers on neuronal cells.