ارزيابي آنتي ژن پروتو اسكولكس اكينو كوكوس گرانولوزوس در تشخيص بيماران مبتلا به كيست هيداتيد با استفاده از روش الايزا و ايمنوبلات

Evaluation of Echinococcus granulosus protoscolex antigen in human cystic echinococcosis detection using ELISA and Western Blotting

Objectives: Serological diagnosis of cystic echinococcosis (CE), caused by Echinococcus granulosus, has been performed by different techniques. The lack of a standardised method for antigen preparation affects the sensitivity and specificity of the tests. The aim of this study was to assess the usefulness of Echinococcus granulosus protoscolex antigen, obtained from naturally infected sheep, using ELBA and immunoblot techniques. Methods: Overall we tested 353 serum samples comprising: one hundred sera from surgically confirmed cystic echinococcosis, 200 sera from native healthy sheep and 53 heterologous sera were evaluated. Results: ELISA results showed 86 of samples of hydatidosis sera were positive and 14 samples showed negative. While 14 samples of heterologous group showed cross reaction. The sensitivity and specificity of ELISA obtained 86% and 75.5% respectively. On the other hand, protoscolex of Echinococcus granulosus, after electerophoresis by SDS-PAGE, showed 13 antigenic bands of 28-112 KDa that reacted with serum samples. With immunoblot test . 93 samples of hydatidosis sera showed positive reaction with antigenic hands and 7 samples were negative. The 38 KDa band showed good immunoblot specificity and 59KDa antigen showed high sensitivity. Overall cystic echinococcosis diagnosis by immunohlotting indicated 93% and 64.1% sensitivity and specificity respectively. Conclusions: The findings from the present study suggest that protoscolex can be considered as a good source of antigenic component in serological cystic Echinococco.c granulosus. Furthermore_ the use of the combination of ELISA and immunoblot techniques for confirmation of laboratory diagnosis of human echinococcosis is advantagous. In addition. 38 KDa antigen is the most specific fraction of protoscolex in immunological tests.