بررسي تغييرات زيرگروه هاي لنفوسيت T در گردش خون محيطي در بيماران مبتلا به بروسلوز حاد و مزمن

A STUDY ON PERIPHERAL BLOOD T-LYMPHOCYTE SUB - SETS ALTERATION IN PATIENTS WITH ACUTE AND CHRONIC BRUCELLOSIS

Brucellosis is caused by brucella species which are intracellular pathogens. In the presence of these pathogens, cell mediated immunity (C.M.I) plays a crucial role in immune responses against the invading pathogens. In the present study alterations of T - lymphocyte sub - sets, which are cellular elements of were investigated. Overall, 71 patients with acute brucellosis and 28 patients with chronic brucellosis took part in this study. Diagnosis of brucellosis was based on case history clinical examination and serological tests. The blood T - lymphocytes with CD;, CD4 and CDs surface markers were enumerated by technique of flow cytometry using specific monoclonal antibodies against these surface markers. The results indicated that the percent of CD3+ T - lymphocytes did not show any significant difference in patients with acute and chronic brucellosis (P=0.131), whereas the percent of CD4+ T-lymphocytes in patients with chronic brucellosis decreased significantly (P-0.007). On the contrary, the percent of CDs+ T - lymphocytes in the blood circulation of patients with chronic brucellosis had increased significantly (P=0.008). These alterations in T lymphocytes sub sets are an indication of severe disorder which this infection had induced in the immune system. This disorder has shown its manifestation by upsetting the balance between CD4+ and CDR+ ratio and probably in suppresion of T - lymphocytes proliferation and function .