ارزيابي آنتي ژنهاي كيست هيداتيك در تشخيص هيداتيدوز به روش الايزاي نقطه اي

Evaluation of hydatid cyst antigens for diagnosis of hydatidosis by dot-ELIZA method

Dot-ELISA is one of the diagnostic methods for hydatidosis that has high susceptibility and known factor. In the present study, five antigens were compared by dot-ELISA for diagnosing hydatid cyst. These antigens were homogenated protoscolex antigen (HPA), hydatid layer antigen (HLA) and antigens of fluid hydatid cyst: crude hydatid fluid antigen (CHFA), autoclaved antigen B (AAB) and boiled antigen B (BAB). TO examin every sample, first the antigens was set on the nitrocellulose membrane. Then, this membrane was placed in bovine albumin serum (BSA) %3 for blocking for an hour. After washing the membrane in PBS, it was placed in the first antibody (definite for infection) for an hour. After re-washing with PBS, it was placed in the second antibody that conjugated with peroxides. Then diamino benzidine (DAB) substrate was used for reading the results that were recognized with color spots in places of antigens. The results show these antigens have different susceptibility and they are specific in dot-ELISA: CHFA (54.8% &39.1%), BAB (54.8% &56.5%), AAB (45.2% & 67.4%), HPA (78.6% &30.4%) and HLA (57.1% &50%). Protoscolex antigen has the highest susceptibility and AAB has the highest attributes. To increasing accuracy in hydatid diagnosis, it is better to test serum samples with a high susceptibility test and then a test may be applied with the higher susceptibility.