تغييرات محتواي آلكالوييد تام، آتروپين و اسكوپولامين در برگ گياه شاهبيزك (Atropa belladonna L) از رويشگاه واز-شمال ايران، در ارتباط با برخي عوامل فنولوژيكي و محيطي

Differences of total alkaloid, atropine and scopolamine contents in leaves of Atropa belladonna L. from Vaz area - north of Iran in relation to some environmental and phenological factors

Alkaloids are variable substances and are classified according to their chemical and biochemical properties. Tropane alkaloids such as atropine and scopolamine which are ornitine derivatives are the most important alkaloids of Atropa belladonna. In this study the plant leaves was gathered from three stations of Vaz-Chamestan area in north of Iran in different seasons. Alkaloids were extracted from leavesʹ powders three times from every sample. Total alkaloid of extracts was determined by UV spectrophotometer (258 nm). HPLC analysis was performed to determine atropine and scopolamine content of samples three times a sample. Soil samples were studied also for texture, pH, EC, etc. The most soil porosity and C/N ratio were observed in 3rd station and the least one in the first station. All of soils were mildly alkaline without salinity. Height of station had significant influence on total alkaloid contents and the common influence of height - season of sampling also was significant. Atropine content always was higher than scopolamine. There was also seen significant correlation between some soil properties and alkaloid content in some cases. Total alkaloid contents had always significant correlation with atropine and scopolamine contents.