تهيه سرم هيپرايميون طاعون گاوي با استفاد ه از ويروس تخفيف حد ت يافته و عاد ت د اد ه شد ه به سلول پرايمري كليه خرگوش و بكارگيري آن د ر تست هاي ژل د يفيوژن و خنثي سازي سرم

Preparation of hyperimmune serum against adaptated plowright rinderpest virus to primary rabbit kidney cell j and using in serum neutralization and agar gel immunodiffusion tests

Hyperimmune sera are used in diagnostic laboratories abundantly. Because of the imported antisera are expensive, the internal preparation with high quality, is very important. In this study, the hyperimmune serum against adaptated plowright rinderpest virus to primary rabbit kidney cell prepared and used in agar gel immunodiffusion test and serum neutralization test. Primary cell, prepared with enzymatic disaggregation method. The attenuated virus was inoculated and the cytopathic effect was seen in 7th blind passage and itʹs ideal assay ( 10ʹ5 ) obtained in 18th passage. The antiserum prepared after injection to rabbit and created clear precipitation lines in agar immunodiffusion test. In SN, the 1 : 64 titer in 100 TCID50 could neutralized the RPV. The sensitivity and specificity of this antiserum was 100% and 80% , that is suitable. At the end, the adaptated virus and hyperimmune serum lyophilized. This serum kept to delivering to the diagnostic laboratories.