سينيتيك اثر برخي داروهاي پايين آورنده فشار خون بر فعاليت آنزيم پراكسيداز

Kinetic Effect of some Antihypertensive Drugs on Peroxidase Activity in-vitro

Peroxidase, EC1.11.1.7, is a heme protein that oxidases various substrates in the presence of hydrogen peroxide. Due to their high catalytic activity and prime role in capture of free radicals, peroxidases are of great importance in research subjects related to medicine and biochemistry. The aim of this research was to investigate the effect of some hypertensive drugs, which are available and commonly used, acting by various mechanisms, on biological activity of peroxidase. Practically, enzyme activity was obtained in the presence of different concentrations of selected hypertensive drugs, atenonol, lozartan and captoprile. The results showed that while atenonol acted as peroxidase activator, lozartan and captoprile reacted as inhibitors of peroxidase. Michaeils constants, Km and Vmax were also measured at IC50 of each drug. The reversibility of reactions was investigated using dialysis processes and it was shown the all three drugs affected enzyme activity reversibly.