پديد آورندگان :
ناقوسي ، حامد نويسنده , , محبي، سيدرضا نويسنده استاديار گروه ويروس‌شناسي ، مركز تحقيقات بيماريهاي گوارش و كبد ، دانشگاه علوم پزشكي شهيد بهشتي Mohebbi, reza , طاهايي، سيد محمد ابراهيم نويسنده كارشناسي ارشد ويروس شناسي، دانشگاه علوم پزشكي شهيد بهشتي مركز تحقيقات گوارش و كبد، محقق، تهران، ايران Tahaei , mohammad ebrahim , عظيم زاده، پدرام نويسنده كارشناس ارشد علوم سلولي و مولكولي، مركز تحقيقات بيماريهاي گوارش و كبد، دانشگاه علوم پزشكي شهيد بهشتي، تهران، ايران , , روماني، سارا نويسنده كارشناس ارشد ميكروبيولوژي، مركز تحقيقات بيماريهاي گوارش و كبد، دانشگاه علوم پزشكي شهيد بهشتي ، تهران، ايران , , صنعتي، آذر نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sanati, Azar , شريفيان ، افسانه نويسنده , , درخشان، فرامرز نويسنده , , زالي ، محمدرضا نويسنده zali, mohammad reza
كليدواژه :
Hepatitis B , IL-12 , Polymorphism , Single nucleotide
چكيده لاتين :
Background: Hepatitis B virus is a major cause of incidence of chronic hepatitis in the world. Although the cause of chronic infection in individuals is not well known, cytokines that are of the main factors to regulate the immune reaction play an important role in determining the clinical course of infection of this virus, and host genetic background and single nucleotide polymorphisms have a decisive effect on the level and performance of cytokines. Thus, the aim of this study is to investigate the relationship between the single nucleotide polymorphisms of 3ʹ noncoding region of IL-12 p40 subunit gene on position +1188, and susceptibility to chronic hepatitis B.
Materials and Methods: This case-control study was performed on a population including 140 patients with chronic hepatitis B and 150 healthy people. PCR-RFLP method was used to determine genotype of individuals. In order to confirm the genotyping results, sequence of 10% of samples was reviewed using direct sequencing.
Results: The frequency of AA, AC and CC genotypes of this gene at position +1188 was respectively calculated 56.4, 36.4 and 7.1% in group of patients and 59.3, 33.3 and 7.3% in the control group. No significant difference was found between both case and control groups (p = 0.487).
Conclusion: The frequency of different genotypes of this gene in the studied population is similar to the results of most studies conducted in different countries. The results of this study shows no association between IL-12B +1188 polymorphism and the risk of chronic hepatitis B is in Iranian population.
