كليدواژه :
زيست شناسي , سلول هاي دندريتيك , PPD , TNF-A , ايمونوتراپي
چكيده لاتين :
Dendritic cells (DCs) with the most powerful antigen-presenting capability possess several clinical applications. Various methods for their generation and maturation are recently under investigation. The purpose of this study was to investigate the efficiency of PPD in maturation induction of monocyte-derived DCs and its comparison with TNF-alpha.
For this purpose, immature dendritic cells were prepared by culturing peripheral blood monocytes from healthy volunteers in the presence of IL-4 and GM-CSF. The obtained immature DCs were stimulated by PPD extract and the results were compared with TNT-a stimulated immature DCs. Morphological characteristics of the cells and their phenotypic markers were used for comparison of the effects of PPD and TNT-a. The expression of CD11 c, CD14, CD1 a, HLA-DR, and CD83 were studied by flow cytometery and their changes were analyzed.
The obtained results showed that the expression of CD14 decreases during culturing period, while CD83 and HLA-DR increases. The changes of CD11c were not statistically significant. In addition, CD 1 a level also relatively decreases during maturation. Regarding the morphological changes, the size of the cells increased by the time and longer cytoplasmic projections was obviously observed on the surface of the cells.
Considering the obtained results, PPD-stimulated dendritic cells showed the phenotype of mature dendritic cells as indicated by high expression of CD83 and HLA-DR and low expression of CDIa. Therefore, similar to TNT-alpha, PPD can induce maturation of DCs and its optimum dose is 150 .miug/ml. Introducing and use of this safe bacterial extract as maturation inducer of DCs can improve the new methods for disease immunotherapy by ex-vivo prepared dendritic cells.
