پديد آورندگان :
سيد شريفي، رئوف نويسنده دانشكده كشاورزي دانشگاه محقق اردبيلي Seyed Sharifi, R. , خرم دل، سرور نويسنده ,
كليدواژه :
باكتري‌هاي PGPR , تلقيح بذر , سويا , نانواكسيد روي
چكيده فارسي :
به‌منظور بررسی تأثیر نانواكسید روی و تلقیح بذر با باكتری‌های افزاینده رشد بر عملكرد، اجزای عملكرد و دوره پر شدن دانه سویا، آزمایشی به‌صورت فاكتوریل در قالب طرح پایه بلوك‌های كامل تصادفی در سه تكرار در مزرعه تحقیقاتی دانشگاه آزاد اسلامی واحد اردبیل در سال 1392 اجرا شد. فاكتورهای مورد بررسی شامل محلول‌پاشی با نانواكسید روی در چهار سطح (صفر، 3/0، 6/0 و 9/0 گرم بر لیتر) و پنج سطح تلقیح (عدم تلقیح بذر به‌عنوان شاهد، تلقیح بذر با برادی رایزوبیوم جاپونیكوم، تلقیح توأم بذر با برادی رایزوبیوم و آزوسپریلیوم لیپوفروم استرین OF، تلقیح توأم بذر با برادی رایزوبیوم و سودوموناس پوتیدا استرین 9، تلقیح بذر با رایزوبیوم+ سودوموناس+ آزوسپریلیوم) بودند. نتایج نشان داد كه با افزایش مصرف نانواكسید روی، وزن خشك گره، تعداد دانه در نیام و تعداد نیام در بوته افزایش یافت. بالاترین وزن خشك گره (05/9 میلی‌گرم)، تعداد نیام در بوته (23/23) و دانه در نیام (08/3) در محلول‌پاشی 9/0 گرم در لیتر نانواكسید روی به‌دست آمد. بالاترین ارتفاع بوته (65/79 سانتی‌متر)، تعداد گره در بوته (55/16)، عملكرد دانه (1875 كیلوگرم در هكتار)، طول دوره پر شدن دانه (76/66 روز)، دوره مؤثر پر شدن دانه (65/54 روز) و سرعت پرشدن دانه (00476/0 گرم در روز) در محلول‌پاشی با نانواكسید روی به میزان 9/0 گرم در لیتر و تلقیح توأم بذر با باكتری‌های محرك رشد و رایزوبیومی به‌دست آمد.
چكيده لاتين :
Introduction
Utilizing biological fertilizer is a proper and cheap method for crop production. Potentially, soybean can be used as biological fertilizers and seed inoculation. Zinc is an essential element that have positive effects on plant growth and its development. Canola, sunflower, soybean and safflower are the main cultivated oilseeds in Iran. Soybean production in Iran is very low as compared to other countries. One of the most effective factor in increasing the soybean yield is seed inoculation with plant growth promoting rhizobacteria (PGPR) and application of Zinc fertilizer. Some of the benefits provided by PGPR are the ability to produce gibberellic acid, cytokinins and ethylene, N2 fixation, solubilization of mineral phosphates and other nutrients (56). Numerous studies have shown a substantial increase in dry matter accumulation and seed yield following inoculation with PGPR. Seyed Sharifi (45) reported that seed inoculation with Azotobacter chroococcum strain 5 increased all of the growth indices such as total dry matter, crop growth rate and relative growth rate. Increasing and extending the role of biofertilizers such as Rhizobium can reduce the need for chemical fertilizers and decrease adverse environmental effects. Therefore, in the development and implementation of sustainable agricultural techniques, biofertilization has great importance in alleviating environmental pollution and deterioration of the nature. As a legume, soybean can obtain a significant portion (4-85%) of its nitrogen requirement through symbiotic N2 fixation when grown in association with effective and compatible Rhizobium strains. Since there is little available information on nano-zinc oxide and seed inoculation by plant growth promoting rhizobacteria (PGPR) on yield in the agro-ecological growing zones of Ardabil province of Iran. Therefore, this research was conducted to investigate the effects of nano-zinc oxide and seed inoculation with plant growth promoting rhizobacteria application on yield, yield components and grain filling period of soybean.
Materials and Methods
In order to study the effects of Nano-Zinc oxide and seed inoculation with Brady rhizobium and plant growth promoting rhizobacteria on yield and some agronomic characteristics of soybean, a factorial experiment based on randomized complete block design with three replications was conducted in 2013 at the research farm of the Islamic Azad University, Ardabil Branch. Factors were included foliar application of Nano-Zinc oxide at four levels (Zero as control, 0.3, 0.6 and 0.9 g l-1) and seed inoculation with Brady rhizobium and plant growth promoting rhizobacteria at five levels (without inoculation as control, seed inoculation with Brady rhizobium japanicum, seed inoculation with Brady rhizobium japanicum+Azosprillum lipoferum strain OF, seed inoculation with Brady rhizobium japanicum+Psedomonas putida, seed inoculation with Brady rhizobium japanicum+ Azosprillum lipoferum strain OF+ Psedomonas putida.
Results and Discussion
The results of growth indices showed that the maximum total dry matter (530 g m-2), crop growth rate (9.48 g.m-2.day-1) and relative growth rate (0.1 g.g-1.day-1) were obtained at foliar application of 0.9 g l-1 Nano-Zinc oxide×seed inoculation with rhizobium+Azosprillum+ Psedomonas and the least of these indices were obtained without of foliar application Nano-Zinc oxide × seed inoculation. The results showed that plant height, the number of nodules per plant, the number of pod per plant, grain yield and grain 100 weight were significantly affected by Nano-Zinc oxide, seed inoculation and interaction of Nano-Zinc oxide×seed inoculation. Maximum of plant height, grain 100 weight, the number of nodules per plant and grain yield were obtained at foliar application of 0.9 g l-1 of Nano-Zinc oxide×seed inoculation with rhizobium and PGPR. Dry weight of nodules per plant, the number of pod per plant and the number of grains per plant inceased by increasing of Nano-Zinc oxide application. Similar results were obtained in seed inoculation with rhizobium+PGPR. In order to increase the grain yield, growth indices and some agronomic characteristics of soybean, it is suggested to apply 0.9 g l-1 of Nano-Zinc oxide and at seed inoculation with rhizobium and PGPR.
Conclusions
Based on the results, it was concluded that application of 0.9 g l-1 of Nano-Zinc oxide and at seed inoculation with rhizobium and PGPR can be recommended for profitable soybean production in the study area.
